THREE NEW VIDEOS ABOUT THE KÜHNER SB10-X BIOREACTOR
In the last weeks, Kühner released three new videos about the orbital shaken benchtop bioreactor SB10-X with single-use bags.The SB10-X is applicable for any type of cell culture process, including perfusion workflows, with human (also stem cells), mammalian, plant, and insect cells. With the standard vessel module, it has a working volume of 4 to 12 liters. The SB10-X is used in research, process development or as a pilot scale bioreactor. Costumers use the bioreactor for instance in the areas of vaccine and antibody production, gene therapy, stem cell cultivation, and artificial meat production. With the 3L-vessel module of the SB10-X, users can perform cell cultivations in single-use bags in small working volumes ranging from 1.5 to 4.5 Liters. The relatively small working volume allows the execution of very cost-efficient perfusion processes since the consumption of costly cell culture media is kept at a necessary minimum.
In the last weeks, Kühner released three new videos about the orbital shaken benchtop bioreactor SB10-X with single-use bags.
The SB10-X is applicable for any type of cell culture process, including perfusion workflows, with human (also stem cells), mammalian, plant, and insect cells. With the standard vessel module, it has a working volume of 4 to 12 liters. The SB10-X is used in research, process development or as a pilot scale bioreactor. Costumers use the bioreactor for instance in the areas of vaccine and antibody production, gene therapy, stem cell cultivation, and artificial meat production.
With the 3L-vessel module of the SB10-X, users can perform cell cultivations in single-use bags in small working volumes ranging from 1.5 to 4.5 Liters. The relatively small working volume allows the execution of very cost-efficient perfusion processes since the consumption of costly cell culture media is kept at a necessary minimum.
How to safely unpack the single-use bag before installing it in the SB10X?
You will find out the answer in the next video. To ensure the proper handling of bag wrappings, it is important to pay attention to the gamma sterilization indicator color, shake the wrapping to confirm the bag is on the bottom, check the location before cutting, and cut a little and tear the rest. Keeping the tag that comes with the bag is also important as it contains vital information. To maintain the bag's sterile condition, Kühner recommends opening it in a sterile environment, ensuring all clamps and couplings are closed before insertion, and checking the sealings for any visible damages. By following these recommendations, your bag will be ready for insertion into the SB10-X.
How to correctly install the single-use bag in the SB10-X?
This video will provide how to set up and use a vessel for harvesting. First, place the bag inside the vessel and insert the harvest tube through the port window. Then, close the position grid and insert the exhaust filter into the designated heater box. Connect the gas supply tube and start the gas mixer to inflate the bag. Secure all tubes with the MagFix removable magnets and connect the optical sensors with the tray module. Ensure that the harvest clamp is as near as possible to the harvest port of the bag to minimize dead volume in the harvest line. Finally, enter the calibration parameters and start the sterile run.
How to install the 3L-vessel module with a single-use bag and start a cultivation?
In this video you can find instructions for assembling and setting up a vessel module for a sterile run/cultivation. The instructions include placing the module on a tray, fixing it with screws, inserting a harvest tube through a port window, managing tubes with mag fixes, filling the bag with air and medium, and connecting fiber glass cables to sensors for DO and pH measurements. Additionally, the video shows calibrating the sensors and starting the sterile run/cultivation.